![]() For this study DNA oligonucleotides were chosen as a model system of biomolecular probes, and fluorescence detection of DNA microarrays served as method of detection. Of all photolinkers and substrates tested, PTD as photolinker and COC as substrate showed the highest photolinking efficiencies and fastest reaction times. We compared the overall photolinking efficiency of all photolinkers with respect to the polymer substrate they are applied to, and we found considerable differences for certain photolinker/substrate combinations. The influence of substrate material is discussed, and three different polymers served as representative substrates: poly(methyl methacrylate) (PMMA), polystyrene (PS), and a cycloolefin copolymer (COC). The influence of these variables are investigated for four prominent photolinkers: ketyl-reactive benzophenone (BP) and anthraquinone (AQ), nitrene-reactive nitrophenyl azide (NPA), and carbene-reactive phenyl-(trifluoromethyl)diazirine (PTD). This study addresses the selection of photolinker and the adjustment of reaction conditions, such as the concentration of biomolecule applied, and irradiation time. Photopolymers, also referred to in the trade as photopolymer resins or UV resins, used for stereolithography/3D printing are based on epoxy and acrylic chemistry.The use of photolinkers (photoactivatable heterobifunctional crosslinkers) is a popular method to attach biomolecules to polymer surfaces. The first photopolymers developed and marketed were based on polyvinyl cinnamates and sensitizers dissolved in solvents and marketed as photoresists by Eastman Kodak Company.Ĭurrently, the fastest growing market for photopolymers is stereolithography/3D printing. However, liquid photoresists may contain a polymer dissolved in a solvent carrier. Liquid compositions such as those used for printing plates and stereolithographic resins usually do not incorporate polymers as a significant component. Polymers usually function as binders and determine processing characteristics as well as final physical properties of cured photopolymer compositions. They may be used along with oligomers, monomers and additives. Polymer bases for photopolymers include acrylics, polyvinyl alcohol, polyvinyl cinnamate, polyisoprene, polyamides, epoxies, polyimides, styrenic block copolymers, nitrile rubber etc. Photopolymer formulations comprise of polymers, oligomers, monomers and additives. A photopolymer product can be applied as a very thin coating as in liquid photoresists or formed into a large model as in a stereolithographic/ 3D printing equipment. Similar liquid compositions can also be used for non-imaging applications such as adhesives, coatings and inks. Immobilization of biomolecules on material surfaces while retaining their biological functions is a major. aryldiazirines) that when irradiated with light turn into reactive intermediates, which bond to all neighboring materials that are either substrate or biomolecule. Photoresists are used to make integrated circuits, flat panel displays, printed circuits, chemically milled parts, MEMS (microelectromechanical systems) etc. This work has designed and synthesised photolinker polymers carrying several photoactivatable chemical functional groups (e.g. A popular use of liquid photopolymers is in making of rubber stamps. which find outlets in printing plates, photoresists, stereolithography/ 3D printing and imaging. ![]() ![]() For final use, they are made into different forms including film/sheet, liquid, solution etc. Photopolymers are imaging compositions based on polymers/oligomers/monomers which can be selectively polymerized and/or crosslinked upon image-wise exposure by light radiation such as ultra-violet light. ![]()
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